MTA, Bio-Oss, and dentin chips have been successfully used in endodontics. The aim of this study was to assess the adhesion and\nmigration of dental stem cells on human pulp ceiling cavities filled with these endodontic materials in an experimental model,\nwhich mimics the clinical conditions of regenerative endodontics. Cavities were formed, by a homemade mold, on untouched\nthird molars, filled with endodontic materials, and observed with electron microscopy. Cells were seeded on cavities� surface\nand their morphology and number were analysed. The phenomenon of tropism was assessed in a migration assay. All three\nmaterials demonstrated appropriate microstructures for cell attachment. Cells grew on all reagents, but they showed a differential\nmorphology.Moreover, variations were observed when comparing cells numbers on cavity�s filling versus the surrounding dentine\ndisc.The highest number of cells was recorded on dentin chips whereas the opposite was true for Bio-Oss. This was confirmed in the\nmigration assay where a statistically significant lower number of cells migrated towards Bio-Oss as compared to MTA and dentin\nchips. This study highlights that MTA and dentin chips have a greater potential compared to Bio-Oss regarding the attraction of\ndental stem cells and are good candidates for bioengineered pulp regeneration.
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